Biological Aging by Trygve O. Tollefsbol
Author:Trygve O. Tollefsbol
Language: eng
Format: epub
Publisher: Humana Press, Totowa, NJ
1.Snap freeze freshly isolated muscle sample in liquid nitrogen-cooled 2-methylbutane in Tissue-Tek OCT. Cut 8 μm muscle cryosections.
2.Air-dry the slide.
3.Fix with 4 % paraformaldehyde for 10 min at room temperature.
4.Wash slides with KPBS 5 min for three times.
5.Block slides with KPBS + 1 % goat serum for 30 min at room temperature.
6.Incubate with KPBSG (KPBS + 0.2 % gelatin) for 5 min.
7.Incubate with primary antibody (1:500 rabbit anti-catalase antibody and 1:400, mouse monoclonal antibody against cytochrome C) in KPBSG + 1 % goat serum for 2 h at room temperature or 4 °C overnight.
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